In the present study, derivative spectrophotometric method in methanol has been developed for the determination of ambroxol in bulk and pharmaceutical dosage drug. The first, second and third order derivative spectroscopy was applied for the estimation of ambroxol using zero-crossing method. Ambroxol exhibits absorption maxima at 248 nm. In the first, second and third derivative spectra of ambroxol the amplitude of positive maxima were measured at 235 nm, 226 nm and 208 nm respectively. Linearity in the concentration range was found to be 10-30 μg/ml. The method were found to be simple economical accurate and reproducible and can be adopted in routine analysis of ambroxol in bulk and dosage form.
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